| 质粒名称: | pBabe EGFR(L858R/T790M) |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 32073 |
| 存储实验室: | Matthew Meyerson |
|
载体骨架基本信息 |
|
| 载体名称: | pBABEpuro |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 5169 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳动物表达，逆转录病毒 |
| 筛选标记: | 嘌呤霉素 |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | EGFR |
| 物种来源: | H. sapiens (human) |
| 插入大小: | 3600 bp |
| 基因突变: | L858R/T790M |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | SnaB1（克隆过程中销毁） |
| 3' 克隆位点: | SnaB1（克隆过程中销毁） |
| 5' 测序引物 | pBABE 5' |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pBabe EGFR(L858R/T790M) was a gift from Matthew Meyerson (Addgene plasmid # 32073 ; http://n2t.net/addgene:32073 ; RRID:Addgene_32073) |
| 参考文献部分: | Allele-dependent variation in the relative cellular potency of distinct EGFR inhibitors. Yuza Y, Glatt KA, Jiang J, Greulich H, Minami Y, Woo MS, Shimamura T, Shapiro G, Lee JC, Ji H, Feng W, Chen TH, Yanagisawa H, Wong KK, Meyerson M. Cancer Biol Ther. 2007 May . 6(5):661-7. 10.4161/cbt.6.5.4003 PubMed 17495523 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
质粒图谱.png)
载体简介
载体序列
LOCUS pBabe EGFR(L858R/T790M) 8725 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pBabe EGFR(L858R/T790M)
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8725)
AUTHORS Yuza Y, Glatt KA, Jiang J, Greulich H, Minami Y, Woo MS, Shimamura
T, Shapiro G, Lee JC, Ji H, Feng W, Chen TH, Yanagisawa H, Wong KK,
Meyerson M
TITLE Allele-dependent variation in the relative cellular potency of
distinct EGFR inhibitors.
JOURNAL Cancer Biol Ther. 2007 May . 6(5):661-7.
PUBMED 17495523
REFERENCE 2 (bases 1 to 8725)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..8725
/organism="synthetic DNA construct"
/mol_type="other DNA"
LTR 1..470
/note="long terminal repeat from Moloney murine leukemia
virus"
misc_feature 535..734
/label=MMLV Psi
/note="packaging signal of Moloney murine leukemia virus
(MMLV)"
CDS 935..1351
/label=gag (truncated)
/note="truncated Moloney murine leukemia virus (MMLV) gag
gene lacking the start codon"
primer_bind 1281..1303
/label=pLXSN 5'
/note="Murine stem cell virus, forward primer. Also called
MSCV"
primer_bind 1319..1335
/label=pBABE 5'
/note="Psi packaging signal, forward primer for pBABE
vectors"
CDS 1379..5011
/codon_start=1
/product="precursor of human epidermal growth factor
receptor"
/label=EGFR
/translation="MRPSGTAGAALLALLAALCPASRALEEKKVCQGTSNKLTQLGTFE
DHFLSLQRMFNNCEVVLGNLEITYVQRNYDLSFLKTIQEVAGYVLIALNTVERIPLENL
QIIRGNMYYENSYALAVLSNYDANKTGLKELPMRNLQEILHGAVRFSNNPALCNVESIQ
WRDIVSSDFLSNMSMDFQNHLGSCQKCDPSCPNGSCWGAGEENCQKLTKIICAQQCSGR
CRGKSPSDCCHNQCAAGCTGPRESDCLVCRKFRDEATCKDTCPPLMLYNPTTYQMDVNP
EGKYSFGATCVKKCPRNYVVTDHGSCVRACGADSYEMEEDGVRKCKKCEGPCRKVCNGI
GIGEFKDSLSINATNIKHFKNCTSISGDLHILPVAFRGDSFTHTPPLDPQELDILKTVK
EITGFLLIQAWPENRTDLHAFENLEIIRGRTKQHGQFSLAVVSLNITSLGLRSLKEISD
GDVIISGNKNLCYANTINWKKLFGTSGQKTKIISNRGENSCKATGQVCHALCSPEGCWG
PEPRDCVSCRNVSRGRECVDKCNLLEGEPREFVENSECIQCHPECLPQAMNITCTGRGP
DNCIQCAHYIDGPHCVKTCPAGVMGENNTLVWKYADAGHVCHLCHPNCTYGCTGPGLEG
CPTNGPKIPSIATGMVGALLLLLVVALGIGLFMRRRHIVRKRTLRRLLQERELVEPLTP
SGEAPNQALLRILKETEFKKIKVLGSGAFGTVYKGLWIPEGEKVKIPVAIKELREATSP
KANKEILDEAYVMASVDNPHVCRLLGICLTSTVQLIMQLMPFGCLLDYVREHKDNIGSQ
YLLNWCVQIAKGMNYLEDRRLVHRDLAARNVLVKTPQHVKITDFGRAKLLGAEEKEYHA
EGGKVPIKWMALESILHRIYTHQSDVWSYGVTVWELMTFGSKPYDGIPASEISSILEKG
ERLPQPPICTIDVYMIMVKCWMIDADSRPKFRELIIEFSKMARDPQRYLVIQGDERMHL
PSPTDSNFYRALMDEEDMDDVVDADEYLIPQQGFFSSPSTSRTPLLSSLSATSNNSTVA
CIDRNGLQSCPIKEDSFLQRYSSDPTGALTEDSIDDTFLPVPEYINQSVPKRPAGSVQN
PVYHNQPLNPAPSRDPHYQDPHSTAVGNPEYLNTVQPTCVNSTFDSPAHWAQKGSHQIS
LDNPDYQQDFFPKEAKPNGIFKGSTAENAEYLRVAPQSSEFIGA"
primer_bind complement(5048..5068)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 5053..5382
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 5233..5368
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 5295..5314
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 5392..5991
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/label=PuroR
/note="confers resistance to puromycin"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
primer_bind complement(5392..5411)
/label=Puro-R
/note="Puromycin resistance gene, reverse primer. Also
called puro-variant-R"
primer_bind 5888..5908
/label=Puro-F
/note="Puromycin resistance gene, forward primer"
LTR 6212..6681
/note="long terminal repeat from Moloney murine leukemia
virus"
primer_bind complement(6811..6828)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(6982..7570)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(7062..7081)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(7730..8590)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 8353..8372
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(8591..8695)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 ctgaatatgg gccaaacagg atatctgtgg taagcagttc ctgccccggc tcagggccaa
61 gaacagatgg aacagctgaa tatgggccaa acaggatatc tgtggtaagc agttcctgcc
121 ccggctcagg gccaagaaca gatggtcccc agatgcggtc cagccctcag cagtttctag
181 agaaccatca gatgtttcca gggtgcccca aggacctgaa atgaccctgt gccttatttg
241 aactaaccaa tcagttcgct tctcgcttct gttcgcgcgc ttctgctccc cgagctcaat
301 aaaagagccc acaacccctc actcggggcg ccagtcctcc gattgactga gtcgcccggg
361 tacccgtgta tccaataaac cctcttgcag ttgcatccga cttgtggtct cgctgttcct
421 tgggagggtc tcctctgagt gattgactac ccgtcagcgg gggtctttca tttgggggct
481 cgtccgggat cgggagaccc ctgcccaggg accaccgacc caccaccggg aggcaagctg
541 gccagcaact tatctgtgtc tgtccgattg tctagtgtct atgactgatt ttatgcgcct
601 gcgtcggtac tagttagcta actagctctg tatctggcgg acccgtggtg gaactgacga
661 gttctgaaca cccggccgca accctgggag acgtcccagg gactttgggg gccgtttttg
721 tggcccgacc tgaggaaggg agtcgatgtg gaatccgacc ccgtcaggat atgtggttct
781 ggtaggagac gagaacctaa aacagttccc gcctccgtct gaatttttgc tttcggtttg
841 gaaccgaagc cgcgcgtctt gtctgctgca gcgctgcagc atcgttctgt gttgtctctg
901 tctgactgtg tttctgtatt tgtctgaaaa ttagggccag actgttacca ctcccttaag
961 tttgacctta ggtcactgga aagatgtcga gcggatcgct cacaaccagt cggtagatgt
1021 caagaagaga cgttgggtta ccttctgctc tgcagaatgg ccaaccttta acgtcggatg
1081 gccgcgagac ggcaccttta accgagacct catcacccag gttaagatca aggtcttttc
1141 acctggcccg catggacacc cagaccaggt cccctacatc gtgacctggg aagccttggc
1201 ttttgacccc cctccctggg tcaagccctt tgtacaccct aagcctccgc ctcctcttcc
1261 tccatccgcc ccgtctctcc cccttgaacc tcctcgttcg accccgcctc gatcctccct
1321 ttatccagcc ctcactcctt ctctaggcgc cggccggatc ccagtgtggt ggtacatcat
1381 gcgaccctcc gggacggccg gggcagcgct cctggcgctg ctggctgcgc tctgcccggc
1441 gagtcgggct ctggaggaaa agaaagtttg ccaaggcacg agtaacaagc tcacgcagtt
1501 gggcactttt gaagatcatt ttctcagcct ccagaggatg ttcaataact gtgaggtggt
1561 ccttgggaat ttggaaatta cctatgtgca gaggaattat gatctttcct tcttaaagac
1621 catccaggag gtggctggtt atgtcctcat tgccctcaac acagtggagc gaattccttt
1681 ggaaaacctg cagatcatca gaggaaatat gtactacgaa aattcctatg ccttagcagt
1741 cttatctaac tatgatgcaa ataaaaccgg actgaaggag ctgcccatga gaaatttaca
1801 ggaaatcctg catggcgccg tgcggttcag caacaaccct gccctgtgca acgtggagag
1861 catccagtgg cgggacatag tcagcagtga ctttctcagc aacatgtcga tggacttcca
1921 gaaccacctg ggcagctgcc aaaagtgtga tccaagctgt cccaatggga gctgctgggg
1981 tgcaggagag gagaactgcc agaaactgac caaaatcatc tgtgcccagc agtgctccgg
2041 gcgctgccgt ggcaagtccc ccagtgactg ctgccacaac cagtgtgctg caggctgcac
2101 aggcccccgg gagagcgact gcctggtctg ccgcaaattc cgagacgaag ccacgtgcaa
2161 ggacacctgc cccccactca tgctctacaa ccccaccacg taccagatgg atgtgaaccc
2221 cgagggcaaa tacagctttg gtgccacctg cgtgaagaag tgtccccgta attatgtggt
2281 gacagatcac ggctcgtgcg tccgagcctg tggggccgac agctatgaga tggaggaaga
2341 cggcgtccgc aagtgtaaga agtgcgaagg gccttgccgc aaagtgtgta acggaatagg
2401 tattggtgaa tttaaagact cactctccat aaatgctacg aatattaaac acttcaaaaa
2461 ctgcacctcc atcagtggcg atctccacat cctgccggtg gcatttaggg gtgactcctt
2521 cacacatact cctcctctgg atccacagga actggatatt ctgaaaaccg taaaggaaat
2581 cacagggttt ttgctgattc aggcttggcc tgaaaacagg acggacctcc atgcctttga
2641 gaacctagaa atcatacgcg gcaggaccaa gcaacatggt cagttttctc ttgcagtcgt
2701 cagcctgaac ataacatcct tgggattacg ctccctcaag gagataagtg atggagatgt
2761 gataatttca ggaaacaaaa atttgtgcta tgcaaataca ataaactgga aaaaactgtt
2821 tgggacctcc ggtcagaaaa ccaaaattat aagcaacaga ggtgaaaaca gctgcaaggc
2881 cacaggccag gtctgccatg ccttgtgctc ccccgagggc tgctggggcc cggagcccag
2941 ggactgcgtc tcttgccgga atgtcagccg aggcagggaa tgcgtggaca agtgcaacct
3001 tctggagggt gagccaaggg agtttgtgga gaactctgag tgcatacagt gccacccaga
3061 gtgcctgcct caggccatga acatcacctg cacaggacgg ggaccagaca actgtatcca
3121 gtgtgcccac tacattgacg gcccccactg cgtcaagacc tgcccggcag gagtcatggg
3181 agaaaacaac accctggtct ggaagtacgc agacgccggc catgtgtgcc acctgtgcca
3241 tccaaactgc acctacggat gcactgggcc aggtcttgaa ggctgtccaa cgaatgggcc
3301 taagatcccg tccatcgcca ctgggatggt gggggccctc ctcttgctgc tggtggtggc
3361 cctggggatc ggcctcttca tgcgaaggcg ccacatcgtt cggaagcgca cgctgcggag
3421 gctgctgcag gagagggagc ttgtggagcc tcttacaccc agtggagaag ctcccaacca
3481 agctctcttg aggatcttga aggaaactga attcaaaaag atcaaagtgc tgggctccgg
3541 tgcgttcggc acggtgtata agggactctg gatcccagaa ggtgagaaag ttaaaattcc
3601 cgtcgctatc aaggaattaa gagaagcaac atctccgaaa gccaacaagg aaatcctcga
3661 tgaagcctac gtgatggcca gcgtggacaa cccccacgtg tgccgcctgc tgggcatctg
3721 cctcacctcc accgtgcaac tcatcatgca gctcatgccc ttcggctgcc tcctggacta
3781 tgtccgggaa cacaaagaca atattggctc ccagtacctg ctcaactggt gtgtgcagat
3841 cgcaaagggc atgaactact tggaggaccg tcgcttggtg caccgcgacc tggcagccag
3901 gaacgtactg gtgaaaacac cgcagcatgt caagatcaca gattttggga gggccaaact
3961 gctgggtgcg gaagagaaag aataccatgc agaaggaggc aaagtgccta tcaagtggat
4021 ggcattggaa tcaattttac acagaatcta tacccaccag agtgatgtct ggagctacgg
4081 ggtgaccgtt tgggagttga tgacctttgg atccaagcca tatgacggaa tccctgccag
4141 cgagatctcc tccatcctgg agaaaggaga acgcctccct cagccaccca tatgtaccat
4201 cgatgtctac atgatcatgg tcaagtgctg gatgatagac gcagatagtc gcccaaagtt
4261 ccgtgagttg atcatcgaat tctccaaaat ggcccgagac ccccagcgct accttgtcat
4321 tcagggggat gaaagaatgc atttgccaag tcctacagac tccaacttct accgtgccct
4381 gatggatgaa gaagacatgg acgacgtggt ggatgccgac gagtacctca tcccacagca
4441 gggcttcttc agcagcccct ccacgtcacg gactcccctc ctgagctctc tgagtgcaac
4501 cagcaacaat tccaccgtgg cttgcattga tagaaatggg ctgcaaagct gtcccatcaa
4561 ggaagacagc ttcttgcagc gatacagctc agaccccaca ggcgccttga ctgaggacag
4621 catagacgac accttcctcc cagtgcctga atacataaac cagtccgttc ccaaaaggcc
4681 cgctggctct gtgcagaatc ctgtctatca caatcagcct ctgaaccccg cgcccagcag
4741 agacccacac taccaggacc cccacagcac tgcagtgggc aaccccgagt atctcaacac
4801 tgtccagccc acctgtgtca acagcacatt cgacagccct gcccactggg cccagaaagg
4861 cagccaccaa attagcctgg acaaccctga ctaccagcag gacttctttc ccaaggaagc
4921 caagccaaat ggcatcttta agggctccac agctgaaaat gcagaatacc taagggtcgc
4981 gccacaaagc agtgaattta ttggagcatg agatgtagga attcgccagc acagtggtcg
5041 accctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga
5101 agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc
5161 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc
5221 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
5281 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag
5341 aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagcttac catgaccgag
5401 tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt acgcaccctc
5461 gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga ccgccacatc
5521 gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag
5581 gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa
5641 gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg
5701 gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg
5761 ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg cagcgccgtc
5821 gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacctcc
5881 gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag
5941 gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg acgcccgccc
6001 cacgacccgc agcgcccgac cgaaaggagc gcacgacccc atgcatcgat aaaataaaag
6061 attttattta gtctccagaa aaagggggga atgaaagacc ccacctgtag gtttggcaag
6121 ctagcttaag taacgccatt ttgcaaggca tggaaaaata cataactgag aatagagaag
6181 ttcagatcaa ggtcaggaac agatggaaca gctgaatatg ggccaaacag gatatctgtg
6241 gtaagcagtt cctgccccgg ctcagggcca agaacagatg gaacagctga atatgggcca
6301 aacaggatat ctgtggtaag cagttcctgc cccggctcag ggccaagaac agatggtccc
6361 cagatgcggt ccagccctca gcagtttcta gagaaccatc agatgtttcc agggtgcccc
6421 aaggacctga aatgaccctg tgccttattt gaactaacca atcagttcgc ttctcgcttc
6481 tgttcgcgcg cttctgctcc ccgagctcaa taaaagagcc cacaacccct cactcggggc
6541 gccagtcctc cgattgactg agtcgcccgg gtacccgtgt atccaataaa ccctcttgca
6601 gttgcatccg acttgtggtc tcgctgttcc ttgggagggt ctcctctgag tgattgacta
6661 cccgtcagcg ggggtctttc acatgcagca tgtatcaaaa ttaatttggt tttttttctt
6721 aagtatttac attaaatggc catagttgca ttaatgaatc ggccaacgcg cgggtagagg
6781 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
6841 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
6901 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
6961 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
7021 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
7081 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
7141 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
7201 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
7261 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
7321 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
7381 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
7441 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
7501 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
7561 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
7621 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
7681 gcggccgcaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
7741 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
7801 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
7861 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
7921 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
7981 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
8041 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
8101 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
8161 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
8221 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
8281 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
8341 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
8401 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
8461 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
8521 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
8581 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
8641 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
8701 ttccccgaaa agtgccacct gcagc
//
